The effect of elevated protein intake on DNA damage in older people: Comparative secondary analysis of two randomized controlled trials
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The effect of elevated protein intake on DNA damage in older people: Comparative secondary analysis of two randomized controlled trials. / Draxler, Agnes; Franzke, Bernhard; Cortolezis, Johannes T; Gillies, Nicola A; Unterberger, Sandra; Aschauer, Rudolf; Zöhrer, Patrick A; Bragagna, Laura; Kodnar, Julia; Strasser, Eva-Maria; Neubauer, Oliver; Sharma, Pankaja; Mitchell, Sarah M; Zeng, Nina; Ramzan, Farha; D’Souza, Randall F; Knowles, Scott; Roy, Nicole Clemence; Sjödin, Anders Mikael; Mitchell, Cameron J; Milan, Amber M; Wessner, Barbara; Cameron-Smith, David; Wagner, Karl-Heinz.
In: Nutrients, Vol. 13, No. 10, 3479, 2021.Research output: Contribution to journal › Journal article › Research › peer-review
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T1 - The effect of elevated protein intake on DNA damage in older people: Comparative secondary analysis of two randomized controlled trials
AU - Draxler, Agnes
AU - Franzke, Bernhard
AU - Cortolezis, Johannes T
AU - Gillies, Nicola A
AU - Unterberger, Sandra
AU - Aschauer, Rudolf
AU - Zöhrer, Patrick A
AU - Bragagna, Laura
AU - Kodnar, Julia
AU - Strasser, Eva-Maria
AU - Neubauer, Oliver
AU - Sharma, Pankaja
AU - Mitchell, Sarah M
AU - Zeng, Nina
AU - Ramzan, Farha
AU - D’Souza, Randall F
AU - Knowles, Scott
AU - Roy, Nicole Clemence
AU - Sjödin, Anders Mikael
AU - Mitchell, Cameron J
AU - Milan, Amber M
AU - Wessner, Barbara
AU - Cameron-Smith, David
AU - Wagner, Karl-Heinz
N1 - CURIS 2021 NEXS 309
PY - 2021
Y1 - 2021
N2 - A high protein intake at old age is important for muscle protein synthesis, however, this could also trigger protein oxidation with the potential risk for DNA damage. The aim of this study was to investigate whether an increased protein intake at recommended level or well above would affect DNA damage or change levels of reduced (GSH) and oxidised glutathione (GSSG) in community-dwelling elderly subjects. These analyses were performed in two randomized intervention studies, in Austria and in New Zealand. In both randomized control trials, the mean protein intake was increased with whole foods, in the New Zealand study (n = 29 males, 74.2 ± 3.6 years) to 1.7 g/kg body weight/d (10 weeks intervention; p < 0.001)) in the Austrian study (n = 119 males and females, 72.9 ± 4.8 years) to 1.54 g/kg body weight/d (6 weeks intervention; p < 0.001)). In both studies, single and double strand breaks and as formamidopyrimidine - DNA glycosylase-sensitive sites were investigated in peripheral blood mononuclear cells or whole blood. Further, resistance to H2O2 induced DNA damage, GSH, GSSG and CRP were measured. Increased dietary protein intake did not impact on DNA damage markers and GSH/GSSG levels. A seasonal-based time effect (p < 0.05), which led to a decrease in DNA damage and GSH was observed in the Austrian study. Therefore, increasing the protein intake to more than 20% of the total energy intake in communitydwelling seniors in Austria and New Zealand did not increase measures of DNA damage, change glutathione status or elevate plasma CRP.
AB - A high protein intake at old age is important for muscle protein synthesis, however, this could also trigger protein oxidation with the potential risk for DNA damage. The aim of this study was to investigate whether an increased protein intake at recommended level or well above would affect DNA damage or change levels of reduced (GSH) and oxidised glutathione (GSSG) in community-dwelling elderly subjects. These analyses were performed in two randomized intervention studies, in Austria and in New Zealand. In both randomized control trials, the mean protein intake was increased with whole foods, in the New Zealand study (n = 29 males, 74.2 ± 3.6 years) to 1.7 g/kg body weight/d (10 weeks intervention; p < 0.001)) in the Austrian study (n = 119 males and females, 72.9 ± 4.8 years) to 1.54 g/kg body weight/d (6 weeks intervention; p < 0.001)). In both studies, single and double strand breaks and as formamidopyrimidine - DNA glycosylase-sensitive sites were investigated in peripheral blood mononuclear cells or whole blood. Further, resistance to H2O2 induced DNA damage, GSH, GSSG and CRP were measured. Increased dietary protein intake did not impact on DNA damage markers and GSH/GSSG levels. A seasonal-based time effect (p < 0.05), which led to a decrease in DNA damage and GSH was observed in the Austrian study. Therefore, increasing the protein intake to more than 20% of the total energy intake in communitydwelling seniors in Austria and New Zealand did not increase measures of DNA damage, change glutathione status or elevate plasma CRP.
KW - Faculty of Science
KW - Protein intake
KW - Elderly
KW - DNA damage
KW - Comet assay
KW - Glutathione
U2 - 10.3390/nu13103479
DO - 10.3390/nu13103479
M3 - Journal article
C2 - 34684481
VL - 13
JO - Nutrients
JF - Nutrients
SN - 2072-6643
IS - 10
M1 - 3479
ER -
ID: 281217459