Identification of molecules derived from human fibroblast feeder cells that support the proliferation of human embryonic stem cells
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Identification of molecules derived from human fibroblast feeder cells that support the proliferation of human embryonic stem cells. / Anisimov, Sergey V.; Christophersen, Nicolaj S.; Correia, Ana S.; Hall, Vanessa Jane; Sandeling, Ingrid; Li, Jia-Yi; Brundin, Patrik.
I: Cellular & Molecular Biology Letters, Bind 16, Nr. 1, 2011, s. 79-88.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Identification of molecules derived from human fibroblast feeder cells that support the proliferation of human embryonic stem cells
AU - Anisimov, Sergey V.
AU - Christophersen, Nicolaj S.
AU - Correia, Ana S.
AU - Hall, Vanessa Jane
AU - Sandeling, Ingrid
AU - Li, Jia-Yi
AU - Brundin, Patrik
PY - 2011
Y1 - 2011
N2 - The majority of human embryonic stem cell lines depend on a feeder cell layer for continuous growth in vitro, so that they can remain in an undifferentiated state. Limited knowledge is available concerning the molecular mechanisms that underlie the capacity of feeder cells to support both the proliferation and pluripotency of these cells. Importantly, feeder cells generally lose their capacity to support human embryonic stem cell proliferation in vitro following long-term culture. In this study, we performed large-scale gene expression profiles of human foreskin fibroblasts during early, intermediate and late passages using a custom DNA microarray platform (NeuroStem 2.0 Chip). The microarray data was validated using RT-PCR and virtual SAGE analysis. Our comparative gene expression study identified a limited number of molecular targets potentially involved in the ability of human neonatal foreskin fibroblasts to serve as feeder cells for human embryonic stem cell cultures. Among these, the C-KIT, leptin and pigment epithelium-derived factor (PEDF) genes were the most interesting candidates.
AB - The majority of human embryonic stem cell lines depend on a feeder cell layer for continuous growth in vitro, so that they can remain in an undifferentiated state. Limited knowledge is available concerning the molecular mechanisms that underlie the capacity of feeder cells to support both the proliferation and pluripotency of these cells. Importantly, feeder cells generally lose their capacity to support human embryonic stem cell proliferation in vitro following long-term culture. In this study, we performed large-scale gene expression profiles of human foreskin fibroblasts during early, intermediate and late passages using a custom DNA microarray platform (NeuroStem 2.0 Chip). The microarray data was validated using RT-PCR and virtual SAGE analysis. Our comparative gene expression study identified a limited number of molecular targets potentially involved in the ability of human neonatal foreskin fibroblasts to serve as feeder cells for human embryonic stem cell cultures. Among these, the C-KIT, leptin and pigment epithelium-derived factor (PEDF) genes were the most interesting candidates.
KW - Former LIFE faculty
KW - Human embryonic stem cells
KW - Feeder cells
KW - DNA microarray
U2 - 10.2478/s11658-010-0039-8
DO - 10.2478/s11658-010-0039-8
M3 - Journal article
C2 - 21161417
VL - 16
SP - 79
EP - 88
JO - Cellular & Molecular Biology Letters
JF - Cellular & Molecular Biology Letters
SN - 1425-8153
IS - 1
ER -
ID: 32438093