Isolation, characterization, and enzymatic hydrolysis of acetyl-galactoglucomannan from spruce (Picea abies)
Publikation: Bidrag til bog/antologi/rapport › Konferencebidrag i proceedings › Forskning › fagfællebedømt
Water-soluble hemicelluloses were extracted from spruce chips by heat-fractionation using microwave treatment. A screening of conditions (pH, temperature and residence time) was performed for the extraction of O-acetyl-galactoglucomannan (AcGGM). The yield and the average molecular weight of the extracted mannan were analysed using HPLC, size-exclusion chromatography (SEC) and mass-spectrometry. The pH during heat fractionation influenced the yield and the structure of AcGGM. The highest yield (78%) of AcGGM (average molecular weight 3800) was achieved with heat-fractionation in water at 190° C for 5 minutes. With 0.025% NaOH, an average molecular weight of 9500 was obtained at a yield of 30%. AcGGM molecular weight standard molecules were prepared using SEC. The structure of the AcGGM was determined using 1H-NMR. The enzymatic hydrolysis of AcGGM was studied using β-mannanase and α-galactosidase.
Originalsprog | Engelsk |
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Titel | Henicelluloses : Science and Technology |
Redaktører | Paul Gatenholm, Maija Tenkanen |
Antal sider | 13 |
Publikationsdato | 1 dec. 2004 |
Sider | 66-78 |
ISBN (Trykt) | 9780841238428 |
Status | Udgivet - 1 dec. 2004 |
Navn | A C S Symposium Series |
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Vol/bind | 864 |
ISSN | 0097-6156 |
ID: 240161930