Bioavailability of seocalcitol IV: evaluation of lymphatic transport in conscious rats
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Bioavailability of seocalcitol IV : evaluation of lymphatic transport in conscious rats. / Grove, Mette; Nielsen, Jeanet L; Pedersen, Gitte P; Müllertz, Anette.
I: Pharmaceutical Research, Bind 23, Nr. 11, 2006, s. 2681-8.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Bioavailability of seocalcitol IV
T2 - evaluation of lymphatic transport in conscious rats
AU - Grove, Mette
AU - Nielsen, Jeanet L
AU - Pedersen, Gitte P
AU - Müllertz, Anette
N1 - Keywords: Animals; Biological Availability; Biological Transport; Calcitriol; Gastrointestinal Tract; Lymph; Rats; Triglycerides
PY - 2006
Y1 - 2006
N2 - PURPOSE: To study the use of long chain triglycerides (LCT) as a lymphotropic carrier of (3)H-seocalcitol by comparing the lymphatic transport and the portal absorption of (3)H-seocalcitol when dissolved in a (1) LCT solution or a (2) reference solution without lipid containing propylene glycol (PG). MATERIALS AND METHODS: A lymph cannulated conscious rat model was dosed orally with (3)H-seocalcitol dissolved in either LCT or PG. Lymph was collected continuously, and blood was sampled over 9 h. (3)H-seocalcitol in blood and lymph and triglycerides in lymph were analysed. RESULTS: A statistically significantly (p < 0.05) higher recovery of the dosed (3)H-seocalcitol was found in the intestinal lymph upon administration of the LCT solution (1.3 +/- 0.6%) compared to the PG solution (0.5 +/- 0.4%). The portal absorption of (3)H-seocalcitol was significantly (p < 0.05) higher from the LCT solution (16.2 +/- 2.2%) than from the PG solution (10.8 +/- 0.8%). CONCLUSIONS: The LCT solution resulted in a statistical significantly higher level of lymphatic and portal transport of (3)H-seocalcitol compared with the PG solution. However, even though LCT facilitates the formation of chylomicrons, (3)H-seocalcitol favours absorption directly to the portal blood probably due to the moderate lipophilicity of the molecule.
AB - PURPOSE: To study the use of long chain triglycerides (LCT) as a lymphotropic carrier of (3)H-seocalcitol by comparing the lymphatic transport and the portal absorption of (3)H-seocalcitol when dissolved in a (1) LCT solution or a (2) reference solution without lipid containing propylene glycol (PG). MATERIALS AND METHODS: A lymph cannulated conscious rat model was dosed orally with (3)H-seocalcitol dissolved in either LCT or PG. Lymph was collected continuously, and blood was sampled over 9 h. (3)H-seocalcitol in blood and lymph and triglycerides in lymph were analysed. RESULTS: A statistically significantly (p < 0.05) higher recovery of the dosed (3)H-seocalcitol was found in the intestinal lymph upon administration of the LCT solution (1.3 +/- 0.6%) compared to the PG solution (0.5 +/- 0.4%). The portal absorption of (3)H-seocalcitol was significantly (p < 0.05) higher from the LCT solution (16.2 +/- 2.2%) than from the PG solution (10.8 +/- 0.8%). CONCLUSIONS: The LCT solution resulted in a statistical significantly higher level of lymphatic and portal transport of (3)H-seocalcitol compared with the PG solution. However, even though LCT facilitates the formation of chylomicrons, (3)H-seocalcitol favours absorption directly to the portal blood probably due to the moderate lipophilicity of the molecule.
KW - Former Faculty of Pharmaceutical Sciences
U2 - 10.1007/s11095-006-9109-z
DO - 10.1007/s11095-006-9109-z
M3 - Journal article
C2 - 17048118
VL - 23
SP - 2681
EP - 2688
JO - Pharmaceutical Research
JF - Pharmaceutical Research
SN - 0724-8741
IS - 11
ER -
ID: 9010043