Intestinal levels of anandamide and oleoylethanolamide in food-deprived rats are regulated through their precursors
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Intestinal levels of anandamide and oleoylethanolamide in food-deprived rats are regulated through their precursors. / Petersen, Gitte; Sørensen, Camilla; Schmid, Patricia C; Artmann, Andreas; Tang-Christensen, Mads; Hansen, Steen H; Larsen, Philip Just; Schmid, Harald H O; Hansen, Harald S.
I: BBA General Subjects, Bind 1761, Nr. 2, 02.2006, s. 143-50; discussion 141-2.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Intestinal levels of anandamide and oleoylethanolamide in food-deprived rats are regulated through their precursors
AU - Petersen, Gitte
AU - Sørensen, Camilla
AU - Schmid, Patricia C
AU - Artmann, Andreas
AU - Tang-Christensen, Mads
AU - Hansen, Steen H
AU - Larsen, Philip Just
AU - Schmid, Harald H O
AU - Hansen, Harald S.
PY - 2006/2
Y1 - 2006/2
N2 - The anorectic lipid oleoylethanolamide and the orexigenic lipid anandamide both belong to the group of N-acylethanolamines that are generated by the enzyme N-acylphosphatidylethanolamine-hydrolyzing phospholipase D. The levels of the two bioactive lipids were investigated in rat intestines after 24 h of starvation as well as after 1 and 4 h of re-feeding. Total levels of precursor phospholipids and N-acylethanolamines were decreased upon food-deprivation whereas the level of the anandamide precursor molecule was significantly increased. The level of 2-arachidonoyl-glycerol was unchanged as was the activity of N-acyltransferase, N-acylphosphatidylethanolamine-hydrolyzing phospholipase D, and fatty acid amide hydrolase upon starvation and re-feeding. It is concluded that remodeling of the amide-linked fatty acids of N-acylphosphatidylethanolamine is responsible for the opposite effects on levels of anandamide and oleoylethanolamide in intestines of food-deprived rats and not an alternative biochemical route for anandamide synthesis. Furthermore, linoleoylethanolamide, which accounted for more than 50 mol% of the endogenous pool of N-acylethanolamines, was found not to have the same inhibitory effect on food intake, as did oleoylethanolamide following oral administration.
AB - The anorectic lipid oleoylethanolamide and the orexigenic lipid anandamide both belong to the group of N-acylethanolamines that are generated by the enzyme N-acylphosphatidylethanolamine-hydrolyzing phospholipase D. The levels of the two bioactive lipids were investigated in rat intestines after 24 h of starvation as well as after 1 and 4 h of re-feeding. Total levels of precursor phospholipids and N-acylethanolamines were decreased upon food-deprivation whereas the level of the anandamide precursor molecule was significantly increased. The level of 2-arachidonoyl-glycerol was unchanged as was the activity of N-acyltransferase, N-acylphosphatidylethanolamine-hydrolyzing phospholipase D, and fatty acid amide hydrolase upon starvation and re-feeding. It is concluded that remodeling of the amide-linked fatty acids of N-acylphosphatidylethanolamine is responsible for the opposite effects on levels of anandamide and oleoylethanolamide in intestines of food-deprived rats and not an alternative biochemical route for anandamide synthesis. Furthermore, linoleoylethanolamide, which accounted for more than 50 mol% of the endogenous pool of N-acylethanolamines, was found not to have the same inhibitory effect on food intake, as did oleoylethanolamide following oral administration.
KW - Animals
KW - Arachidonic Acids
KW - Eating
KW - Endocannabinoids
KW - Food Deprivation
KW - Intestines
KW - Male
KW - Oleic Acids
KW - Phospholipids
KW - Polyunsaturated Alkamides
KW - Rats
KW - Rats, Sprague-Dawley
KW - Appetite Regulation
KW - Former Faculty of Pharmaceutical Sciences
U2 - 10.1016/j.bbalip.2005.12.011
DO - 10.1016/j.bbalip.2005.12.011
M3 - Journal article
C2 - 16478679
VL - 1761
SP - 143-50; discussion 141-2
JO - B B A - General Subjects
JF - B B A - General Subjects
SN - 0304-4165
IS - 2
ER -
ID: 44796934